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Infectious Diseases — Parasitology

Hookworm disease

Hookworm is an intestinal helminthiasis caused by hematophagous nematodes of the Ancylostoma and Necator genus, principally Ancylostoma duodenale and Necator americanus. These worms parasitize the human small intestine, where they attach themselves to the duodenal and jejunal mucosa via their mouth capsules, equipped with hooks or sharp blades, causing chronic mucosal haemorrhaging and leading to daily blood and iron loss. Hookworm is one of the most widespread parasitic infections in the world, affecting between 500 and 700 million people according to the World Health Organization, mainly in the tropical and subtropical regions of sub-Saharan Africa, South and South-East Asia, Latin America and the Caribbean, where inadequate sanitation and barefoot contact with soil contaminated by human faeces are the main routes of transmission. In Quebec and Canada, the disease is almost exclusively observed in recent immigrants, refugees or travellers returning from endemic zones. Its main clinical consequence is chronic, sometimes profound, iron-deficiency anemia, which is particularly deleterious in young children, pregnant women and the malnourished, where it can lead to stunted growth, impaired cognitive development and significant morbidity and mortality.

Pathogen and parasitic cycle

Understanding the hookworm's biological cycle is essential for grasping the modes of contamination, clinical presentation, and prevention strategies. The cycle takes place partly in the soil and partly in the human organism, with a larval migration phase through several tissues before permanent establishment in the small intestine.

Species Geographic distribution Special features
Necator americanus Dominant species in Latin America, Sub-Saharan Africa, Southeast Asia Buccal capsule with sharp plates; blood loss of 0.03 to 0.05 mL per worm per day; adult worm 5 to 11 mm
Hookworm (Old World) Predominant in the Mediterranean, Middle East, South Asia, North Africa Buccal capsule with chitinous hooks; greater blood loss of 0.1 to 0.4 mL per worm per day; can also infect orally or transmammally
Ancylostoma ceylanicum Southeast Asia, Oceania Zoonotic species (dogs, cats) capable of infecting humans; increasingly recognized as a cause of human hookworm infection in certain regions
Ancylostoma braziliense / caninum Tropical and subtropical zones, including the Caribbean and South America Animal species (dogs, cats) causing cutaneous larva migrans in humans (dead-end host) without established intestinal infection

The parasitic cycle begins with the excretion of unembryonated eggs in the feces of an infected host. In warm (15 to 35°C), moist, and shaded soil, eggs hatch in 24 to 48 hours to produce rhabditiform larvae (L1), which transform into infective filariform larvae (L3) in 5 to 10 days. These L3 larvae, capable of surviving in the soil for several weeks, actively penetrate intact skin (primarily the soles of the feet and interdigital regions) upon direct contact with contaminated soil. They then migrate via the bloodstream to the lungs, cross the alveoli, ascend the bronchial tree to the pharynx, are swallowed, and establish themselves in the duodenum and proximal jejunum, where they mature into adults in 4 to 6 weeks. Adult worms can survive for 1 to 5 years in the human intestine, with each worm laying several thousand eggs per day.

Active transcutaneous penetration by L3 larvae is the main route of infection, making wearing shoes on potentially contaminated soil the most effective individual preventive measure. Ancylostoma duodenale can also infect orally through ingestion of larvae on contaminated vegetation or water, and transmammary in infants breastfed by a parasitized mother.

Clinical manifestations

The clinical presentation of hookworm infection evolves in several phases corresponding to the different stages of the parasite's cycle in the body. The severity of the manifestations depends directly on the parasitic load (number of adult worms present in the intestine) and the host's prior nutritional status.

Phase Delay Events
Skin penetration (hookworm dermatitis) A few hours to 48 hours after exposure Intense itching and erythema at the site of larval penetration (usually the soles of the feet or interdigital spaces); transient erythematous papules or vesicles, known as "miner's itch"; spontaneous regression within a few days.
Pulmonary migration (Löffler's syndrome) 1 to 3 weeks after intercourse Dry cough, wheezing, dyspnea, mild hemoptysis, moderate fever, marked blood eosinophilia; transient pulmonary infiltrates on chest X-ray; spontaneous resolution in 2 to 4 weeks
Early intestinal phase (worm establishment) 4 to 8 weeks after contamination Epigastric pain, nausea, vomiting, sometimes bloody diarrhea, flatulence; manifestations are more pronounced during a massive primary infection
Chronic phase (hemorrhagic loss) Several months to years without treatment Progressive iron deficiency anemia (main manifestation): chronic fatigue, paleness, exertional dyspnea, palpitations, headaches, dizziness; hypoalbuminemia due to protein-losing enteropathy; dependent edema in severe forms; pica (ingestion of earth, clay, ice) sometimes observed
The severity of iron-deficiency anemia in hookworm infection is directly proportional to the parasitic load. Mild infection (less than 100 worms) is often asymptomatic in a well-nourished adult. A load of 500 to 1000 worms can lead to moderate to severe anemia, potentially fatal in an already malnourished child or a pregnant woman. It is estimated that each adult hookworm of *Necator americanus* causes a loss of 0.03 to 0.05 mL of blood per day, which can amount to a loss of up to 50 mL per day in heavy infections.

Populations at particular risk

Certain groups have an increased vulnerability to hookworm complications due to their higher iron needs, more limited reserves, or lower tolerance to anemia.

  • Children under 5 years of age and of school age: severe anemia that can lead to stunted growth, impaired cognitive and attention development, decreased school performance, and increased susceptibility to other infections
  • Pregnant women: anemia increases the risk of prematurity, low birth weight, maternal mortality, and insufficient iron transfer to the fetus; hookworm infestation is a major preventable cause of anemia during pregnancy in endemic areas.
  • People already deficient in iron or protein: insufficient reserves accelerate the development of clinically significant anemia even with moderate parasitic loads
  • First-generation immigrants and refugees from endemic areas: may be chronic asymptomatic carriers and present with unexplained iron deficiency anemia requiring systematic parasitological screening
  • Travelers on extended stays in precarious hygiene conditions: risk of contamination from walking barefoot on soiled floors, gardening, or recreational ground activities in tropical endemic zones.

Diagnosis

The diagnosis of hookworm infection is based on the detection of parasite eggs in the stool, supplemented by biological tests to assess the hematological and nutritional impact of the infection.

Review Expected results Diagnostic value
Stool parasitological examination (SPE) Highlighting of hookworm eggs: oval, thin-walled, containing 2 to 8 cells (depending on the freshness of the sample); 40 to 60 µm long Reference examination; improved sensitivity with the Kato-Katz technique also allowing for quantification of parasitic load (eggs per gram of stool); 3 samples from different days recommended to increase sensitivity
Complete blood count (CBC) Microcytic hypochromic anemia (iron deficiency); variable eosinophilia depending on the phase (marked in migratory larval phase, less so in established chronic intestinal phase) Orient towards diagnosis and assess anemia severity; eosinophilia associated with iron deficiency anemia should suggest intestinal parasitosis.
Martial assessment Low serum iron, low or depleted ferritin, high transferrin (increased TIBC), decreased transferrin saturation coefficient Confirm the iron-deficiency nature of the anemia and assess the extent of iron depletion.
Albuminemia and total protein Hypoalbuminemia, hypoproteinemia in severe forms with protein-losing enteropathy Marker of overall nutritional severity and impact; dependent edema if albuminemia is below 25 g/L
Kato-Katz Technique (quantitative) Egg count per gram of stool: mild infection less than 1999 eggs/g, moderate 2000 to 3999, severe greater than 4000 Allows for the classification of infection severity and guides therapeutic and public health decisions; recommended by the WHO for mass deworming programs
Serology and fecal PCR Non-specific serological tests (cross-reactions between helminths); fecal PCR available in some specialized laboratories with high sensitivity and specificity PCR is useful for mild infections below the microscopic detection threshold or for species differentiation (N. americanus vs. A. duodenale); not routinely available in Quebec.
Any unexplained iron deficiency anemia in a recent immigrant, refugee, or traveler returning from a tropical or subtropical area should systematically prompt a stool parasitological examination with a concentration technique, even in the absence of digestive symptoms. Chronic hookworm infection can persist for years with few symptoms, manifesting solely as iron deficiency anemia resistant to oral iron supplementation without antiparasitic treatment.

Differential diagnosis

Ancylostomiasis can be mistaken for other conditions, particularly when skin or pulmonary manifestations are predominant, or when iron-deficiency anemia is isolated without an obvious epidemiological context.

Affection Distinctive elements
Cutaneous larva migrans (hookworm larvae) Serpiginous pruritic subcutaneous tract progressing a few millimeters per day; parasitic impasse in humans without intestinal involvement or anemia; treated with ivermectin or albendazole
Other intestinal helminthiases (ascariasis, trichuriasis, strongyloidiasis) Distinction by parasitological examination of stool; strongyloidiasis is particularly important to distinguish because it can lead to fatal hyperinfection in immunocompromised individuals.
Iron-deficiency anemia of other cause Dietary deficiency, chronic digestive bleeding (ulcer, colorectal cancer), malabsorption (celiac disease, Crohn's disease); EPS and epidemiological context suggest hookworm infection.
Löffler's syndrome of other causes Ascariasis in the pulmonary larval stage, toxocariasis, filariasis, drug reaction (DRESS); context and examinations guide the diagnosis
Contact allergy or eczema at the site of penetration No travel to endemic areas, non-migratory clinical presentation, different chronic evolution; traveler's history is determinant

Treatment

Hookworm treatment is antiparasitic, supplemented by correction of iron-deficiency anemia and associated nutritional deficiencies. The efficacy of available anthelmintics is excellent, and parasitological cure is achieved in the vast majority of cases after a single dose or short treatment.

Treatment Terms and conditions Remarks
Albendazole (standard treatment) 400 mg orally in a single dose for adults and children over 2 years of age; a 3-day course (400 mg/day) for severe infections or resistant forms Cure rate of 72 to 95 % depending on species and studies; slightly higher against Ancylostoma duodenale vs Necator americanus; to be taken with a fatty meal to improve absorption; contraindicated in the first trimester of pregnancy
Mebendazole (alternative) 100 mg twice a day for 3 days, or 500 mg in a single dose Slightly lower cure rate than albendazole (60-79 %); bioavailability varies with food intake; available and inexpensive; also contraindicated in the first trimester
Pyrantel Pamoate (alternative) 11 mg/kg (maximum 1 g) in a single dose, or for 3 days for severe infections Option in case of allergy or unavailability of benzimidazoles; comparable efficacy but less commonly used in practice
Ivermectin 200 µg/kg in a single dose Modest efficacy against hookworms compared to benzimidazoles; interesting in cases of coinfection with *Strongyloides stercoralis* or filaria, which are frequent in the same endemic areas.
Iron Supplementation Ferrous sulfate 150 to 200 mg of elemental iron per day in 2 to 3 doses, for 3 to 6 months depending on the severity of the deficiency Essential alongside antiparasitic treatment to correct iron deficiency anemia; parasite elimination alone without supplementation is insufficient to replenish depleted reserves; take on an empty stomach or with vitamin C for better absorption
Folic acid supplementation 5 mg/day in pregnant women and malnourished children Frequently associated with iron deficiency in malnourished populations in endemic areas; essential in pregnant women to prevent fetal complications
Red blood cell transfusion Indicated in case of severe symptomatic anemia (hemoglobin below 70 g/L with poor clinical tolerance) or severe anemia in pregnant women Reserved for forms with hemodynamic decompensation or vital risk; not a substitute for antiparasitic treatment and correction of deficiencies
A parasitological stool examination is recommended 2 to 4 weeks after the end of antiparasitic treatment to confirm cure. In case of treatment failure or persistent eggs, a second course of treatment or the use of a combination of anthelmintics may be considered. In patients with regular contact with endemic areas (migrant workers, frequent travelers), the risk of reinfection warrants prolonged vigilance and periodic screening.

Prevention

Individual prevention relies on simple but very effective behavioral measures. Community prevention in endemic areas involves coordinated public health programs combining mass treatment, improved sanitation, and health education.

  • Wearing closed shoes at all times in at-risk areas: the most effective individual measure to prevent transdermal penetration of L3 larvae on contaminated soil
  • Avoid walking barefoot or lying directly on the ground in tropical and subtropical areas, especially on beaches, lawns, or garden soil frequented by dogs or cats.
  • Careful washing of hands and raw vegetables when staying in endemic areas.
  • Do not consume untreated water or vegetables irrigated with wastewater in resource-limited countries
  • Preventive deworming considered in travelers with prolonged contact with contaminated soil in endemic areas, according to the travel doctor's assessment
  • Systematic parasitological screening is recommended for recent immigrants, refugees, and adopted children from endemic areas, even in the absence of symptoms
  • WHO-led mass drug administration (MDA) programs in high-prevalence endemic areas, targeting school-aged children and pregnant women through annual or semi-annual administration of albendazole or mebendazole

Consult at Clinique Omicron

Clinique Omicron welcomes patients in its Quebec branches for all health-related questions, including post-travel parasitic screenings, recent immigrant screenings, and the management of unexplained iron deficiency anemias. Our physicians provide initial clinical evaluations, prescribe appropriate follow-up tests, and refer patients to infectious disease or travel medicine specialists when necessary. Book an appointment at the branch nearest you.

The content of this page is provided for informational purposes only and is not intended to replace the advice of a qualified healthcare professional. Consult a physician for any symptoms, questions or decisions you may have regarding your health.

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